Rejuvenome leverages single nuclei RNA-seq as a workhorse assay to profile the effects of anti-aging interventions in mice throughout the body. Given that, it is imperative for us to find cost-effective solutions for library preparation and sequencing. In a later post we will discuss our ongoing comparison between Parse Biosciences and 10x Genomics kits for […]
In a previous post we explained why we had opted to work with outbred—genetically diverse—mice, narrowing down our options to either the UM-HET3 or Diversity Outbred (DO) stocks. Our first pilot involved purchasing a small batch of DO mice (20 males, 20 females) to see whether we could work with these mice in practice. Now […]
The workhorse of mammalian biomedical research is a mouse strain known as C57BL/6. These mice are isogenic: they are all genetically identical. In addition to that, these mice are also homozygous at all sites of their genomes. This is achieved by crossing the mice with each other, brother-to-sister, a process called inbreeding. In an ideal […]
Overarching Goals and Structure The Rejuvenome is designed to uncover the effects of aging-interventions (treatments targeting the biological processes of aging) throughout the entire body, spanning molecular and cellular changes to functional differences of individual organs and the body as a whole. For robustness, the study will use genetically diverse mice that more closely resemble […]
What can we do to most improve human health? How can we help people live longer, healthier lives? Several things come to mind: eradicating infectious disease, eliminating poverty and homelessness, protecting the environment. There are many efforts in these areas, and more are urgently needed. But what is not widely understood is that the process […]
Welcome to the Rejuvenome Blog! Here we will share updates on the progress of the project, present results, discuss some key decisions made on the experimental design, and more. Rejuvenome has a mandate to help the aging field as a whole by producing comprehensive atlases of the effects of various aging interventions in aged, genetically […]